Unexpected complexation reaction during analysis of proteins using laser desorption spray post-ionization mass spectrometry.
نویسندگان
چکیده
We describe complexation reactions of insulin and other proteins with metal ions generated from the substrate surface by laser irradiation in laser desorption spray post-ionization mass spectrometry (LDSPI-MS). This particular type of complexation reaction in LDSPI-MS was investigated for the first time, which indicated that the mechanistic process of LDSPI-MS might be much more complicated than that proposed before for similar methods.
منابع مشابه
Rapid trace detection of triacetone triperoxide (TATP) by complexation reactions during desorption electrospray ionization.
Desorption electrospray ionization (DESI) mass spectrometry is used for rapid, specific and sensitive detection of trace amounts of the notorious explosive TATP present on ambient surfaces by alkali metal complexation in a simple spray technique.
متن کاملAnalysis of the Photo Conversion of Asphaltenes Using Laser Desorption Ionization Mass Spectrometry: Fragmentation, Ring Fusion, and Fullerene Formation
The conversion or photo conversion of asphaltenes to polycyclic aromatic hydrocarbons (PAH’s) promoted by a laser source is analyzed using both experimental and theoretical methods. We propose that during measurements performed at an intermediate laser power, fragmentation to afford PAH’s and ring fusion to yield fused PAH’s (FPAH’s) may occur either within molecular clusters (resin case) or wi...
متن کاملMALDI-TOF MS Coupled with Collision-Induced Dissociation (CID) Measurements of Poly(methyl methacrylate)
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) is one of themost fundamental analysis techniques for proteins, peptides, and synthetic polymers to date. Along with field desorption (FD), fast atom bombardment (FAB), laser desorption coupled with time-of-flight (LD-TOF), secondary ion mass spectrometry coupled with time-of-flight (SIMS-TOF), and electr...
متن کاملcis-Diol functional group recognition by reactive desorption electrospray ionization (DESI).
Heterogeneous reactions at a solution/solid interface are utilized in an ambient mass spectrometry experiment to recognize the cis-diol functionality by its selective complexation reaction to form a cyclic boronate.
متن کاملCoupling desorption electrospray ionization with ion mobility/mass spectrometry for analysis of protein structure: evidence for desorption of folded and denatured States.
A desorption electrospray ionization (DESI) source has been coupled to an ion mobility time-of-flight mass spectrometer for the analysis of proteins. Analysis of solid-phase horse heart cytochrome c and chicken egg white lysozyme proteins with different DESI solvents and conditions shows similar mass spectra and charge state distributions to those formed when using electrospray to analyze these...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Analyst
دوره 137 8 شماره
صفحات -
تاریخ انتشار 2012